{"id":18378,"date":"2023-02-21T13:29:06","date_gmt":"2023-02-21T13:29:06","guid":{"rendered":"https:\/\/www.ttci-th.org\/?p=18378"},"modified":"2023-11-28T14:26:41","modified_gmt":"2023-11-28T14:26:41","slug":"3d-cell-models","status":"publish","type":"post","link":"https:\/\/www.ttci-th.org\/th\/3d-cell-models\/","title":{"rendered":"\u0e41\u0e1a\u0e1a\u0e08\u0e33\u0e25\u0e2d\u0e07\u0e40\u0e0b\u0e25\u0e25\u0e4c 3 \u0e21\u0e34\u0e15\u0e34\u0e43\u0e2b\u0e49\u0e04\u0e27\u0e32\u0e21\u0e23\u0e39\u0e49\u0e43\u0e2b\u0e21\u0e48\u0e40\u0e01\u0e35\u0e48\u0e22\u0e27\u0e01\u0e31\u0e1a\u0e21\u0e30\u0e40\u0e23\u0e47\u0e07\u0e17\u0e48\u0e2d\u0e19\u0e49\u0e33\u0e14\u0e35 \u0e21\u0e2d\u0e1a\u0e04\u0e27\u0e32\u0e21\u0e2b\u0e27\u0e31\u0e07\u0e2a\u0e33\u0e2b\u0e23\u0e31\u0e1a\u0e01\u0e32\u0e23\u0e23\u0e31\u0e01\u0e29\u0e32\u0e17\u0e35\u0e48\u0e14\u0e35\u0e02\u0e36\u0e49\u0e19"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"18378\" class=\"elementor elementor-18378\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b9af823 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no\" data-id=\"b9af823\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2db16c3\" data-id=\"2db16c3\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e57f26e elementor-widget elementor-widget-text-editor\" data-id=\"e57f26e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.16.0 - 14-09-2023 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#69727d;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#69727d;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>Recent advancements in cholangiocarcinoma (CCA) research have been marked by the innovative use of three-dimensional multicellular spheroid (3D MCS) models. These models, which replicate the 3D architecture and multicellular arrangement of human tissues, are proving to be more physiologically relevant than traditional 2D cultures in studying this form of cancer.<\/p><p>Key findings from this research reveal that the ability of CCA cell lines to form 3D MCSs varies based on their differentiation level. Poorly differentiated CCA cell lines struggle to form 3D MCSs due to a deficiency in cell adhesion molecules and a higher expression of mesenchymal markers. In contrast, well-differentiated CCA and cholangiocyte cell lines successfully develop 3D MCSs characterized by round shapes and smooth perimeters, facilitated by the presence of cell adhesion molecules.<\/p><p>A pivotal discovery in this study is the formation of a hypoxic and oxidative microenvironment within these 3D structures. This was particularly evident in the spheroids formed by MMNK-1, KKU-213C, and KKU-213A cell lines. Proteo-metabolomic analysis of these 3D MCSs revealed significant differences in protein expression and metabolic products compared to their 2D cultured counterparts. Notably, there were alterations in cell-cell adhesion molecules, enzymes related to energy metabolism, and metabolites associated with oxidative stress.<\/p><p>These findings underscore the physiological differences between 3D MCSs and traditional 2D cultures, highlighting the former&#8217;s potential in providing a more accurate representation of the in vivo state. The study suggests that the 3D MCS model could lead to the identification of alternative biochemical pathways, which may be crucial for developing more effective treatments for CCA.<\/p><p>This research paves the way for a better understanding of the complex molecular and metabolic landscape of cholangiocarcinoma, and holds promise for the development of targeted therapies that could significantly improve patient outcomes.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d777e2d elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no\" data-id=\"d777e2d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-fbc0aee\" data-id=\"fbc0aee\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f42f7db elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"f42f7db\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.16.0 - 14-09-2023 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">\u0e40\u0e07\u0e34\u0e19\u0e17\u0e38\u0e19<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a3809f5 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"a3809f5\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.16.0 - 14-09-2023 *\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#0c0d0e;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:\"\";border-bottom:0;flex-grow:1;border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right .elementor-divider__element{margin-right:0}.elementor-widget-divider:not(.elementor-widget-divider--view-line_text):not(.elementor-widget-divider--view-line_icon) .elementor-divider-separator{border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--separator-type-pattern{--divider-border-style:none}.elementor-widget-divider--separator-type-pattern.elementor-widget-divider--view-line .elementor-divider-separator,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e1c8b36 elementor-widget elementor-widget-text-editor\" data-id=\"e1c8b36\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>This research was supported by a grant from the National Research Council of Thailand through Fluke Free Thailand Project and Cholangiocarcinoma Screening and Care Program, Khon Kaen University (CASCAP-18), CARI-04\/2561, and the NSRF under the Basic Research Fund of Khon Kaen University (CARI-BRF64-26) through Cholangiocarcinoma Research Institute allocated to Poramate Klanrit. We thank the Faculty of Medicine, Khon Kaen University, Thailand, for the Invitation Research Grant (IN64327) allocated to Miss Pimpawadee Phukhum. We also appreciated the valuable consultation and discussion with Khon Kaen University Phenome Centre (KKUPC) on metabolomic results.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a3c7bb6 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no\" data-id=\"a3c7bb6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e51aa36\" data-id=\"e51aa36\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-61b459f elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"61b459f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\n\u0e01\u0e23\u0e30\u0e14\u0e32\u0e29\u0e15\u0e49\u0e19\u0e09\u0e1a\u0e31\u0e1a<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e2c38ec elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"e2c38ec\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f7592ce elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f7592ce\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>\u0e0a\u0e37\u0e48\u0e2d\u0e1a\u0e17\u0e04\u0e27\u0e32\u0e21\u0e15\u0e49\u0e19\u0e09\u0e1a\u0e31\u0e1a:<\/strong> The impact of hypoxia and oxidative stress on proteo-metabolomic alterations of 3D cholangiocarcinoma models<br \/><strong>\u0e27\u0e32\u0e23\u0e2a\u0e32\u0e23: <\/strong>\u0e15\u0e31\u0e27\u0e41\u0e17\u0e19\u0e27\u0e34\u0e17\u0e22\u0e4c<span class=\"anchor-text\"><br \/><\/span><strong>\u0e14\u0e2d\u0e22:<\/strong> <a href=\"https:\/\/www.nature.com\/articles\/s41598-023-30204-y\">10.1038\/s41598-023-30204-y<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6a0c5b0 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no\" data-id=\"6a0c5b0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-8b1e9bf\" data-id=\"8b1e9bf\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-42ed5b4 elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"42ed5b4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u0e08\u0e14\u0e2b\u0e21\u0e32\u0e22\u0e42\u0e15\u0e49\u0e15\u0e2d\u0e1a<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9157dcb elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"9157dcb\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-14cd458 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"14cd458\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><a href=\"https:\/\/www.researchgate.net\/profile\/Poramate-Klanrit-4\">\u0e1b\u0e23\u0e40\u0e21\u0e29\u0e10\u0e4c \u0e01\u0e25\u0e31\u0e48\u0e19\u0e24\u0e17\u0e18\u0e34\u0e4c<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5b74787 elementor-section-boxed elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no\" data-id=\"5b74787\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-29ff5c2\" data-id=\"29ff5c2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-12e396c elementor-widget elementor-widget-jkit_social_share\" data-id=\"12e396c\" data-element_type=\"widget\" data-widget_type=\"jkit_social_share.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div  class=\"jeg-elementor-kit jkit-social-share jeg_module___69e4fd2e4c131\" ><ul class=\"social-share-list\"><li class=\"elementor-repeater-item-14d1b21\" data-social=\"facebook\">\n\t\t\t\t<a class=\"facebook social-icon\"><span class=\"icon-position-before\"><i aria-hidden=\"true\" class=\"fab fa-facebook-f\"><\/i>\u0e40\u0e1f\u0e2a\u0e1a\u0e38\u0e4a\u0e04<\/span><\/a>\n\t\t\t<\/li><li class=\"elementor-repeater-item-ee9a855\" data-social=\"twitter\">\n\t\t\t\t<a class=\"twitter social-icon\"><span class=\"icon-position-before\"><i aria-hidden=\"true\" class=\"fab fa-twitter\"><\/i>\u0e17\u0e27\u0e34\u0e15\u0e40\u0e15\u0e2d\u0e23\u0e4c<\/span><\/a>\n\t\t\t<\/li><li class=\"elementor-repeater-item-4a6d796\" data-social=\"line\">\n\t\t\t\t<a class=\"line social-icon\"><span class=\"icon-position-before\"><i aria-hidden=\"true\" class=\"fab fa-line\"><\/i>\u0e40\u0e2a\u0e49\u0e19<\/span><\/a>\n\t\t\t<\/li><\/ul><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Recent advancements in cholangiocarcinoma (CCA) research have been marked by the innovative use of three-dimensional multicellular spheroid (3D MCS) models. These models, which replicate the 3D architecture and multicellular arrangement of human tissues, are proving to be more physiologically relevant than traditional 2D cultures in studying this form of cancer. Key findings from this research&#8230;<\/p>","protected":false},"author":4,"featured_media":18383,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[106],"tags":[100],"class_list":["post-18378","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research-news-research-news-2","tag-tag-topic-ku"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/posts\/18378","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/comments?post=18378"}],"version-history":[{"count":0,"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/posts\/18378\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/media\/18383"}],"wp:attachment":[{"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/media?parent=18378"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/categories?post=18378"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ttci-th.org\/th\/wp-json\/wp\/v2\/tags?post=18378"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}